Potential for diagnosis versus therapy monitoring of attention deficit...
AbstractIn view of the need for easily accessible biomarkers, we evaluated in ADHD children the epigenetic status of the 5′-untranslated region (UTR) in the SLC6A3 gene, coding for human dopamine...
View ArticleThe endocannabinoid hydrolase FAAH is an allosteric enzyme
AbstractFatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive...
View ArticleAuthor Correction: The endocannabinoid hydrolase FAAH is an allosteric enzyme
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
View ArticleClik here to view.
Phytocannabinoids and endocannabinoids: different in nature
AbstractCannabis is one of the earliest cultivated plants, of which Cannabis sativa and Cannabis indica are the most widespread and best characterized species. Their extracts contain...
View ArticleRegulation of oxytocin receptor gene expression in obsessive–compulsive...
AbstractBackgroundObsessive–compulsive disorder (OCD) is a prevalent and severe clinical condition. Robust evidence suggests a gene-environment interplay in its etiopathogenesis, yet the underlying...
View ArticleRadiometric Assay of NAPE-PLD Activity
AbstractN-Acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides, a family of bioactive lipids including...
View ArticleRadiometric Assay of FAAH Activity
AbstractFatty acid amide hydrolase (FAAH) is an intracellular enzyme responsible for the hydrolysis of endogenous anandamide (AEA), a reaction that terminates the biological effects of this lipid...
View ArticleEndocannabinoid Metabolism and Transport as Drug Targets
AbstractThe wide distribution of the endocannabinoid system (ECS) throughout the body and its pivotal pathophysiological role offer promising opportunities for the development of novel therapeutic...
View ArticleDNA–Protein-Interaction (DPI)-ELISA Assay for PPAR-γ Receptor Binding
AbstractDysregulation of peroxisome proliferator-activated receptor (PPAR)-γ has been described in a plethora of pathological conditions, such as diabetes, obesity, inflammatory-related diseases, and...
View ArticleVisualization of Endocannabinoids in the Cell
AbstractA still unsolved, although critical, issue in endocannabinoid research is the mechanism by which the lipophilic anandamide (AEA) moves from its site of synthesis, crosses the aqueous milieu,...
View ArticleRadiometric Assay of ABHD2 Activity
AbstractThe α,β-hydrolase fold-containing protein 2 (ABHD2) is a serine hydrolase, responsible for the cleavage of endogenous 2-arachidonoylglycerol (2-AG). ABHD2 is activated by progesterone, thus, it...
View ArticleFluorimetric Assay of FAAH Activity
AbstractFatty acid amide hydrolase (FAAH) is the enzyme responsible for the degradation of anandamide (N-arachidonoylethanolamine, AEA) to arachidonic acid (AA) and ethanolamine. The method described...
View ArticleNeed for Methods to Investigate Endocannabinoid Signaling
AbstractEndocannabinoids (eCBs) are endogenous lipids able to bind to cannabinoid receptors, the primary molecular targets of the cannabis (Cannabis sativa) active principle Δ9-tetrahydrocannabinol....
View ArticleTRPV1 channels are critical brain inflammation detectors and neuropathic pain...
AbstractThe capsaicin receptor TRPV1 has been widely characterized in the sensory system as a key component of pain and inflammation. A large amount of evidence shows that TRPV1 is also functional in...
View ArticleHuman osteogenic differentiation in Space: proteomic and epigenetic clues to...
AbstractIn the frame of the VITA mission of the Italian Space Agency (ASI), we addressed the problem of Space osteoporosis by using human blood-derived stem cells (BDSCs) as a suitable osteogenic...
View ArticleThe anti-inflammatory agent bindarit acts as a modulator of fatty...
AbstractWe investigated the cellular and molecular mechanisms by which bindarit, a small indazolic derivative with prominent anti-inflammatory effects, exerts its immunoregulatory activity in...
View ArticleCannabinoid signalling and effects of cannabis on the male reproductive system
AbstractMarijuana is the most widely consumed recreational drug worldwide, which raises concerns for its potential effects on fertility. Many aspects of human male reproduction can be modulated by...
View Article